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Zeiss inverted microscope
| Scanning stage XYZ
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- Travel range 130mmx85mm
- Travel range in Z: 13mm
- Max speed 100mm/s
- Resolution: 0.1um
- Absolute accuracy: +/- 5um
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| Z-piezo stage
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- Fast and precise movement in the Z axis
- Z-range: 250 micrometer
- Frequency: up to 90 Hz
- Min step size: 10nm
- Max load: 100g
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| Objectives
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- EC Plan-Neofluar 10x/0.3
- LCI Plan-Neofluar 25x/0.8 Imm. Corr. DIC (for oil, water or glycerine immersion)
- Plan-Apochromat 40x/1.3 Oil DIC
- Plan-Apochromat 63x/1.40 Oil DIC
- Plan-Apochromat 100x/1.46 Oil DIC
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| DIC sliders
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- LCI PN 25x/0.8 II
- PA 40x/1.3 III
- PA 63x/1.4 HC II
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| Filters
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- Zeiss Set 43 Cy3, d=25 shift free
- Zeiss Set 38 Endow GFP shift free
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| Condensers
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- LD condenser 0.55, for brightfield, phase contrast and DIC
- Achromatic condenser 0.8 for brightfield and DIC
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| Transmitted light illuminator
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| Reflected light illuminator
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- Fl Illumination System X-Cite 120 XL FL PC, 120W metal halogenide lamp
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Zeiss digital camera
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- Resolution: 5 Megapixels
- Dynamic range: 1-1300
- Fast live imaging
- Binning: 1x1 to 10x10 for increased sensitivity
- Colour depth: 36 bit RGB
- Interface: standard FireWire
- Large field of view, 2/3‘‘ CCD
- Trigger In/Out signals for control of external components
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Zeiss confocal microscope
| Detector
| 34-channel spectral detection
- A filter-free detection unit with a 32-channel array detector and two side PMTs
- Imaging of up to 10 channels in Channel Mode or 34 channels in Lambda Mode
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| Laser sources
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- Argon multiline laser source 25mW (458, 488, 514nm)
- HeNe Laser 543nm
- Wavelength range: 488 - 640 nm
- Power: > 1.5 mW
- Repetition rate: 40 MHz
- Pulse length: < 5 ps
- Tuning speed: < 50 nm / s
- Bandwidth: < 3 nm
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Zeiss multi-photon imaging
| Laser source
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| Detectors
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- Non- Descanned Detectors in the Reflectied Light Path: 2 channels filterset green/red (SP500-550/BP565-610)
- Non- Descanned Detectors in the Transmitted Light Path: filterset green/red (SP500-550/BP565-610)
- Transmission PhotoMultiplier Detector (T-PMT)
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Zeiss confocal system optimized for fast live scanning
| Live Scanner
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- Scan resolution: Up to 1536 x 1536 pixels
- Scanning speed: Variable up to 120 frames/s with 512 x 512 pixels; faster modes with smaller frames(e.g. 505 frames/s with 512 x 100 pixels, 1010 frames/s with 512 x 50 pixels); ultrafast line scan mode with > 60,000 lines/s
- Scan zoom: 0.5x to 2.0x, digital
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| Laser sources
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- Argon laser 488nm, 100mW
- Laser line 532nm, 75mW
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Fujitsu Siemens computer
| Workstation
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- CPU: Intel Xeon X5260 @ 3.33Ghz
- RAM: 4.00GB
- OS: 64-bit Windows Vista Ultimate
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Zeiss software
| Analysis Tools in ZEN
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- Linear Unmixing
- Histogram
- Profile Histogram
- Colocalization Analysis
- 3D Rendering
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| Additional Software modules
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- FRAP Analysis
- FRET Analysis
- RICS: Raster Image Correlation Spectroscopy
- Anisotropy
- Measurement in 3D
- Deconvolution
- Mean of ROI: Measures Changes in Intensities over Time (Photomanipulation)
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