Zeiss inverted microscope  
Scanning stage XYZ
  • Travel range 130mmx85mm
  • Travel range in Z: 13mm
  • Max speed 100mm/s
  • Resolution: 0.1um
  • Absolute accuracy: +/-  5um


Z-piezo stage
  • Fast and precise movement in the Z axis
  • Z-range: 250 micrometer
  • Frequency: up to 90 Hz
  • Min step size: 10nm
  • Max load: 100g


Objectives
  • EC Plan-Neofluar 10x/0.3
  • LCI Plan-Neofluar 25x/0.8 Imm. Corr. DIC (for oil, water or glycerine immersion)
  • Plan-Apochromat 40x/1.3 Oil DIC
  • Plan-Apochromat 63x/1.40 Oil DIC
  • Plan-Apochromat 100x/1.46 Oil DIC


DIC sliders
  • LCI PN 25x/0.8 II
  • PA 40x/1.3 III
  • PA 63x/1.4 HC II


Filters
  • Zeiss Set 43 Cy3, d=25 shift free
  • Zeiss Set 38 Endow GFP shift free


Condensers
  • LD condenser 0.55, for brightfield, phase contrast and DIC
  • Achromatic condenser 0.8 for brightfield and DIC


Transmitted light illuminator
  • HAL 100 illuminator



Reflected light illuminator
  • Fl Illumination System X-Cite 120 XL FL PC, 120W metal halogenide lamp


Zeiss digital camera
  • Resolution: 5 Megapixels
  • Dynamic range: 1-1300
  • Fast live imaging
  • Binning: 1x1 to 10x10 for increased sensitivity
  • Colour depth: 36 bit RGB
  • Interface: standard FireWire  
  • Large field of view, 2/3‘‘ CCD
  • Trigger In/Out signals for control of external components



Zeiss confocal microscope    
Detector
34-channel spectral detection    
  • A filter-free detection unit with a 32-channel array detector and two side PMTs    
  • Imaging of up to 10 channels in Channel Mode or 34 channels in Lambda Mode


Laser sources
  • Argon multiline laser source 25mW (458, 488, 514nm)
  • HeNe Laser  543nm

  • Wavelength range: 488 - 640 nm
  • Power: > 1.5 mW
  • Repetition rate: 40 MHz
  • Pulse length: < 5 ps
  • Tuning speed: < 50 nm / s
  • Bandwidth: < 3 nm



Zeiss multi-photon imaging
Laser source  


Detectors
  • Non- Descanned Detectors in the Reflectied Light Path: 2 channels filterset green/red (SP500-550/BP565-610)
  • Non- Descanned Detectors in the Transmitted Light Path: filterset green/red (SP500-550/BP565-610)
  • Transmission PhotoMultiplier Detector (T-PMT)



Zeiss confocal system optimized for fast live scanning  
Live Scanner  
  • Scan resolution: Up to 1536 x 1536 pixels
  • Scanning speed: Variable up to 120 frames/s with 512 x 512 pixels; faster modes with smaller frames(e.g. 505 frames/s with 512 x 100 pixels, 1010 frames/s with 512 x 50 pixels); ultrafast line scan mode with > 60,000 lines/s
  • Scan zoom: 0.5x to 2.0x, digital


Laser sources
  • Argon laser 488nm, 100mW
  • Laser line 532nm, 75mW



Fujitsu Siemens computer
Workstation
  • CPU:   Intel Xeon X5260 @ 3.33Ghz
  • RAM:   4.00GB
  • OS:     64-bit Windows Vista Ultimate



Zeiss software
Analysis Tools in ZEN
  • Linear Unmixing
  • Histogram
  • Profile Histogram
  • Colocalization Analysis
  • 3D Rendering


Additional Software modules
  • FRAP Analysis
  • FRET Analysis
  • RICS: Raster Image Correlation Spectroscopy
  • Anisotropy
  • Measurement in 3D
  • Deconvolution
  • Mean of ROI: Measures Changes in Intensities over Time (Photomanipulation)